TransTaq® DNA Polymerase High Fidelity (HiFi) (with 2.5 mM dNTPs)
Cat# AP131-12
Size : 500units
Brand : TransGen Biotech
ManualTransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
• TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.
Applications
• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP131-01/11 | AP131-02/12 | AP131-03/13 |
| TransTaq® HiFi DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransTaq® HiFi Buffer I | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 10×TransTaq® HiFi Buffer II | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 2.5 mM dNTP | -/400 μl×1 | -/800 μl×1 | -/800 μl×6 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
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