TransScript® First-Strand cDNA Synthesis SuperMix
Cat# AT301-03
Size : 100rxns×20ulsystem
Brand : TransGen Biotech
ManualThe product uses RNA as a template to efficiently synthesize the first-strand cDNA by TransScript® RT/RI Enzyme Mix and
2×TS Reaction Mix. It is easy to operate and can reduce contamination risk.
Features
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
• cDNA up to 12 kb.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AT301-02 | AT301-03 |
| TransScript® RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×TS Reaction Mix | 500 μl | 1 ml |
| Random Primer (N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo (dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
Li S, Tian Y, Wu K, et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2018, 560(7720):595-600.
Wang S , Wu K , Qian Q , et al. Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield[J]. Cell Research, 2017, 27(9):1142-1156.
Ye J , Ge J , Zhang X , et al. Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds[J]. Cell Research, 2016, 26(1):34-45.
Xu H, Lin Z, Li F, et al. Dimerization of elongator protein 1 is essential for Elongator complex assembly[J]. Proc Natl Acad Science U S A. 2015, 112(34):10697-702.
Wang K , Hou W D , Wang X , et al. Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane[J]. Biomaterials, 2016, 102:249-258.