Thymidine [50-89-5]
Cat# HY-N1150-1g
Size : 1g
Brand : MedChemExpress
Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.
For research use only. We do not sell to patients.
Thymidine Chemical Structure
CAS No. : 50-89-5
This product is a controlled substance and not for sale in your territory.
Based on 7 publication(s) in Google Scholar
Other Forms of Thymidine:
- Thymidine-d3 In-stock
- Thymidine-d4 In-stock
- Thymidine-13C10,15N2 In-stock
- Thymidine-15N2 In-stock
- Thymidine-13C5 In-stock
- Thymidine-d14 Get quote
- Thymidine-13C10 Get quote
- Thymidine-13C Get quote
- Thymidine-13C-1 Get quote
- Thymidine-13C-2 Get quote
- Thymidine-2′-13C Get quote
- Thymidine-d2 Get quote
Description |
Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3]. |
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IC50 & Target[2] |
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In Vivo |
Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4+Vβ8+ and CD8+Vβ8+ T cells [3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Clinical Trial |
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Molecular Weight |
242.23 |
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Formula |
C10H14N2O5 |
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CAS No. |
50-89-5 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O[C@H]1C[C@H](N2C(NC(C(C)=C2)=O)=O)O[C@@H]1CO |
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Structure Classification |
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Initial Source |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 50 mg/mL (206.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 33.33 mg/mL (137.60 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: 99.96% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 4.1283 mL | 20.6415 mL | 41.2831 mL | 103.2077 mL |
5 mM | 0.8257 mL | 4.1283 mL | 8.2566 mL | 20.6415 mL | |
10 mM | 0.4128 mL | 2.0642 mL | 4.1283 mL | 10.3208 mL | |
15 mM | 0.2752 mL | 1.3761 mL | 2.7522 mL | 6.8805 mL | |
20 mM | 0.2064 mL | 1.0321 mL | 2.0642 mL | 5.1604 mL | |
25 mM | 0.1651 mL | 0.8257 mL | 1.6513 mL | 4.1283 mL | |
30 mM | 0.1376 mL | 0.6881 mL | 1.3761 mL | 3.4403 mL | |
40 mM | 0.1032 mL | 0.5160 mL | 1.0321 mL | 2.5802 mL | |
50 mM | 0.0826 mL | 0.4128 mL | 0.8257 mL | 2.0642 mL | |
60 mM | 0.0688 mL | 0.3440 mL | 0.6881 mL | 1.7201 mL | |
80 mM | 0.0516 mL | 0.2580 mL | 0.5160 mL | 1.2901 mL | |
100 mM | 0.0413 mL | 0.2064 mL | 0.4128 mL | 1.0321 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Thymidine Related Classifications
- Cancer
- Cancer Targeted Therapy Cancer Immunotherapy Cancer Metabolism and Metastasis
- Cell Cycle/DNA Damage Metabolic Enzyme/Protease Anti-infection
- DNA/RNA Synthesis Endogenous Metabolite Orthopoxvirus