Reagents and instruments for immunology, cell biology and molecular biology.
 
> SynaptoRed™C2

SynaptoRed™C2

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Cat# 70027

Size : 5x1MG

Brand : Biotium


SynaptoRed™ C2 (Equivalent to FM®4-64)

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

SynaptoRed™ C2 (equivalent to FM®4-64)

Product Attributes

CAS number

162112-35-8
Probe cellular localization

Membrane/vesicular
For live or fixed cells

For live/intact cells
Assay type/options

Real-time imaging
Colors

Far-red
Excitation/Emission

510/750 nm (see product description)

Technology

Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
SDS
Supporting Documents

Product Description

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia. Please also see AM4-64 (70025), which is similar to SynaptoRed™, but with a formaldehyde-fixable amine group.

SynaptoRed™ can also be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λEx/λEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • C30H45Br2N3
  • MW: 608
  • [162112-35-8]

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed C2 (FM®4-64) (Ref. 4).

Nerve Terminal Dyes and Kits

Product Catalog No. Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)		 70020... 70053 View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)		 70021... 70050 View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-7 70029 Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS 70037 Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 101 80101 Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I 70030 Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A) 70031 Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B) 70031-1 Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III 70032 Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V 70034 Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
 

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