Retinol Binding Protein (Cellular retinol-binding protein I, CRBP1, CRBP2, RBP, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma) (Biotin)

Cat# 168860-AF-Biotin-100ul

Size : 100ul

Brand : US Biological

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168860-AF-Biotin Retinol Binding Protein (Cellular retinol-binding protein I, CRBP1, CRBP2, RBP, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P02753, P09455, P50120 (Human); Q00915 (Mouse); P02696 (Rat)
Isotype
IgG1,k
Grade
Purified
Applications
IF IHC IP WB
Crossreactivity
Gt Hu Mk Mo Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.||Applications: |Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 1-2ug/ml |Immunoprecipitation: 1-2ug/500ug protein|Western Blot: 0.5-1ug/ml|Immunohistochemistry: Frozen only: 1-2ug/ml for 30 min at RT|Optimal dilutions to be determined by the researcher.||Positive Control:|Liver or placenta||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: G4E4|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: Retinol binding protein purified from human plasma. Cellular Localization: Cytoplasmic|Specificity: Recognizes human Retinol Binding Protein. Species Crossreactivity: monkey, goat, rabbit, rat, and mouse Retinol Binding Protein. Others-not tested.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Retinol binding protein purified from human plasma. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Retinol Binding Protein. Species Crossreactivity: monkey, goat, rabbit, rat, and mouse Retinol Binding Protein. Others-not tested.
References
1. Reddy BM; Karande AA; Adiga PR. Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies. Molecular Immunology, 1993, 30(15):1355-60.