The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 -20 class="pro-form-title">Product Properties
Kit components
μg. % SDS, 5% Triton X-100, 5% Tween-20, 60, 80 in the sample. to 2000 -2,000 . Use a microplate reader to measure the absorbance at 562 nm, or the absorbance at other wavelengths between 540-595 nm. . The protein concentration determined by BCA method is not affected by chemical substances in most samples, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween20, 60, 80 in the sample. However, the BCA method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no EGTA, dithiothreitol (DTT) is less than 1 mM, and β-mercaptoethanol (β-Mercaptoethanol) is less than 0.01%. . It is recommended to make a standard curve for each measurement. Because the color of the BCA method will continue to deepen with the extension of time, and the color reaction will be accelerated due to the increase of temperature. . If the sample diluent or lysate itself has a high background, please try the Protein Quantification Kit (Bradford Assay) (Cat #:KTD3002).
Storage instructions
Store at room temperature, stable for 12 months
Shipping
Room temperature transport
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis.
Additional Information
Background
Abbkine Protein Quantification Kit (BCA Assay) is improved on the basis of BCA method, one of the commonly used protein concentration detection methods in the world. The principle of this method is that the protein reduces copper ions (Cu2+) to cuprous ions (Cu+ ) in alkaline conditions, and the generated Cu+ forms a purple complex with BCA, which has a strong absorption peak at 562 nm, and the absorbance value It is proportional to the protein content in the sample, and the protein concentration can be calculated according to the absorbance value.
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