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> PNPP (disodium) [4264-83-9]

PNPP (disodium) [4264-83-9]

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Cat# HY-15928-1g

Size : 1g

Brand : MedChemExpress


PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

For research use only. We do not sell to patients.

PNPP disodium Chemical Structure

PNPP disodium Chemical Structure

CAS No. : 4264-83-9

This product is a controlled substance and not for sale in your territory.

Based on 3 publication(s) in Google Scholar

Other Forms of PNPP disodium:

  • 4-Nitrophenyl phosphate disodium hexahydrate In-stock
  • 4-Nitrophenyl phosphate Get quote
  • 4-Nitrophenyl phosphate ditromethamine Get quote
  • 4-Nitrophenyl phosphate cyclohexylammonium Get quote
Description

PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

In Vitro

Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PNPP disodium Related Antibodies
Molecular Weight

263.05

Formula

C6H4NNa2O6P
CAS No.

4264-83-9
Appearance

Solid

Color

White to yellow

SMILES

O=P(O[Na])(O[Na])OC1=CC=C([N+]([O-])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (608.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8016 mL 19.0078 mL 38.0156 mL
5 mM 0.7603 mL 3.8016 mL 7.6031 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (380.16 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.85%

COA (254 KB) HNMR (153 KB) LCMS (348 KB)

Handling Instructions (2659 KB)
References

  • [1]. Crowther JR, et al. Evaluation of the use of chromogenic and fluorogenic substrates in solid-phase enzyme linked immunosorbent assays (ELISA). Biologicals. 1990 Oct;18(4):331-6.  [Content Brief]

  • [1]. Crowther JR, et al. Evaluation of the use of chromogenic and fluorogenic substrates in solid-phase enzyme linked immunosorbent assays (ELISA). Biologicals. 1990 Oct;18(4):331-6.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.8016 mL 19.0078 mL 38.0156 mL 95.0390 mL
5 mM 0.7603 mL 3.8016 mL 7.6031 mL 19.0078 mL
10 mM 0.3802 mL 1.9008 mL 3.8016 mL 9.5039 mL
15 mM 0.2534 mL 1.2672 mL 2.5344 mL 6.3359 mL
20 mM 0.1901 mL 0.9504 mL 1.9008 mL 4.7519 mL
25 mM 0.1521 mL 0.7603 mL 1.5206 mL 3.8016 mL
30 mM 0.1267 mL 0.6336 mL 1.2672 mL 3.1680 mL
40 mM 0.0950 mL 0.4752 mL 0.9504 mL 2.3760 mL
50 mM 0.0760 mL 0.3802 mL 0.7603 mL 1.9008 mL
60 mM 0.0634 mL 0.3168 mL 0.6336 mL 1.5840 mL
80 mM 0.0475 mL 0.2376 mL 0.4752 mL 1.1880 mL
100 mM 0.0380 mL 0.1901 mL 0.3802 mL 0.9504 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PNPP disodium Related Classifications

  • Others
  • Others
  • Fluorescent Dye
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PNPP disodium4264-83-9Para-nitrophenyl phosphate disodiumFluorescent DyeInhibitorinhibitorinhibit

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