Reagents and instruments for immunology, cell biology and molecular biology.

p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Ser/Thr Phosphatase Assay Kit, BioAssay™

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Cat# N2600-35-1Kit

Size : 1Kit

Brand : US Biological

N2600-35 Rabbit Anti-p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Ser/Thr Phosphatase Assay Kit, BioAssay™

Clone Type

Polyclonal

Swiss Prot

P30154

Shipping Temp

Blue Ice

Storage Temp

4°C

Intended Use: |The kit is used to detect PP2A activity by either dephosphorylation of the phosphopeptide (R-K- pT-I-R-R) or hydrolysis of pNPP.||Kit Components:|N2600-35A: Malachite Green (Solution A), 1x20ml |N2600-35B: Malachite Green Additive (Solution B), 1x500ul |N2600-35C: Phosphate Standard, 1mM (Solution C), 1x1ml |N2600-35D: p-Nitrophenyl Phosphate (pNPP), 2x5mg tablets|N2600-35E: Phosphopeptide (KRpTIRR), 1x1mg|N2600-35F: NiCl2, 40mM, 1x1ml |N2600-35G: pNPP Ser/Thr Assay Buffer (50mM Tris-HCl, pH 7.0, 100uM CaCl2), 1x20ml |N2600-35H: Microtiter Plate, 1x96 wells||Storage and Stability:|Store at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Harder, K.W., et al., Biochem. J. 298: 395-401 (1994). 2. Ambach, A., et al., Eur. J. Immunol. 30: 3422-3431 (2000). 3. Santoro, M., et al., J. Biol. Chem. 273: 13,119-13,128 (1998). 4. Pankov, R., et al., J. Biol. Chem. 278: 18,671-18,681 (2003).