65194 | Mouse Monoclonal Anti-Synaptopodin, supernatant [Clone G1D4]

anti-Synaptopodin/SYNPO mouse monoclonal, G1D4, supernatant

Key Features

Hybridoma cell culture supernatant
Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with human, mouse and rat
Isotype: IgG1

Product description

Quantity	5 ml
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG1
Conjugate	Unconjugated
Application	ICC/IF, IHC, WB
Purification	Hybridoma cell culture supernatant
Reactivity	Human, Mouse, Rat
No reactivity	Chicken, Frog, Rabbit
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	G1D4
Immunogen	Isolated rat kidney glomeruli
Formulation	Contains 0.09% sodium azide
UniprotID	A4IFK4 (Bovine), A0A286XK19 (Guinea pig), Q8N3V7 (Human), Q91YE8 (Mouse), D4A702 (Rat)
Synonym	Synaptopodin, SYNPO, KIAA1029

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (IHC) - frozen	1:50-1:200
Immunohistochemistry (IHC) - paraffin	1:50-1:200 (microwave treatment recommended)
Western Blot (WB)	Assay dependent

Background

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kDa and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kDa, pI 9.27. In SDS-PAGE the antigen appears as 100 kDa polypeptide in brain and 110 kDa polypeptide in kidney (the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kDa degradation fragment of synaptopodin.

References/Publications (46)

Publication

Species

Application

Publication Moritz Schanz Martin Kimmel Mark Dominik Alscher Kerstin Amann Christoph Daniel., TIMP-2 and IGFBP7 in human kidney biopsies in renal disease. Clin Kidney J, 16 1434-1446, (2023).

Species Human

Application IHC-IF

Publication Montandon, M., Hamidouche, T., et al. Telomerase is required for glomerular renewal in kidneys of adult mice. NPJ Regen Med. (2022).

Species Mouse

Application IHC-IF

Publication Zhu, Z. et al. Mitoquinone Protects Podocytes from Angiotensin II-Induced Mitochondrial Dysfunction and Injury via the Keap1-Nrf2 Signaling Pathway. Oxid. Med. Cell. Longev. 2021, (2021).

Species rat

Application IHC-IF

Publication Ekulu, P. M. et al. Novel human podocyte cell model carrying g2/g2 apol1 high-risk genotype. Cells 10, (2021).

Species human

Application WB

Publication Tsuchida, Y. et al. Lipoprotein modulation of proteinuric renal injury. Lab.Invest. , (2019)

Species mouse

Application IHC

Publication Ornellas, F. et al. Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model. Sci.Rep. 9, 19604 (2019)

Species rat

Application IHC (paraffin)

Publication An, J. et al. Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis. Sci.Rep. 9, 14707 (2019)

Species human

Application WB

Publication Toffoli, B. et al. Hemicentin 1 influences podocyte dynamic changes in glomerular diseases. Am.J.Physiol.Renal.Physiol. 314, F1154-F1165 (2018)

Species mouse

Application IHC-IF (paraffin)

Publication Held, M. et al. Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS.One. 13, e0199918 (2018)

Species human

Application ICC-IF

Publication Schell, C. et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 47, 741-757.e8(2018).

Species mouse

Application IHC (paraffin)

Publication Rachubik, P. et al. The TRPC6-AMPK pathway is involved in insulin-dependent cytoskeleton reorganization and glucose uptake in cultured rat podocytes. Cell. Physiol. Biochem. 51, 393–410 (2018).

Species Rat

Publication Zhou, J. et al. Functional comparison of distinct Brachyury+ states in a renal differentiation assay. Biol.Open. 7,- (2018).

Species mouse

Application IHC-IF (frozen)

Publication Prochnicki, A. et al. Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat. Am.J.Pathol. 188, 2529-2541 (2018)

Species rat

Application IHC-IF (paraffin)

Publication Manigrasso, M. et al. Deletion of the formin Diaph1 protects from structural and functional abnormalities in the murine diabetic kidney. Am.J.Physiol.Renal.Physiol. 315, F1601-F1612 (2018)

Species mouse

Application IHC (paraffin)

Publication Haley, K. et al. Podocyte injury elicits loss and recovery of cellular forces. Sci.Adv. 4, eaap8030 (2018).

Species human

Application ICC

Publication Endlich, N. et al. The transcription factor Dach1 is essential for podocyte function. J.Cell.Mol.Med. 22, 2656-2669 (2018).

Species human,mouse

Application ICC

Publication Armelloni, S. et al. NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites. Cell.Physiol.Biochem. 46, 873-889 (2018)

Species human,mouse

Application IHC-IF (paraffin)

Publication Gauer, S. et al. CTGF Is Expressed During Cystic Remodeling in the PKD/Mhm (cy/+) Rat Model for Autosomal-Dominant Polycystic Kidney Disease (ADPKD). J.Histochem.Cytochem. 65, 743-755 (2017)

Species rat

Application IHC (paraffin)

Publication Fukuda, A. et al. Urinary podocyte and TGF-?1 mRNA as markers for disease activity and progression in anti-glomerular basement membrane nephritis. Nephrol.Dial.Transplant. 32, 1818-1830 (2017).

Species human

Application IHC (paraffin)

Publication Jain, N. et al. TLR-mediated albuminuria needs TNF?-mediated cooperativity between TLRs present in hematopoietic tissues and CD80 present on non-hematopoietic tissues in mice. Dis. Model. Mech. 9, 707-17 (2016).

Species mouse

Application IHC (frozen)

Publication Canales, C. et al. Characterization of a Trpc6 Transgenic Mouse Associated with Early Onset FSGS. Br. J. Med. Med. Res. 5, 1198–1212 (2015).

Species mouse

Application IHC-IF

Publication Wang, L. et al. Gq signaling causes glomerular injury by activating TRPC6. J. Clin. Invest. 125, 1913-26 (2015).

Species mouse

Application WB,IHC (frozen)

Publication Ihara, K. I. et al. MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney. Am. J. Pathol. 184, 2699-2708 (2014).

Species mouse

Application IHC (frozen)

Publication Tanaka, E. et al. Notch2 activation ameliorates nephrosis. Nat. Commun. 5, 3296 (2014).

Species mouse

Application IHC (frozen)

Publication Puliti, A. et al. Albuminuria and Glomerular Damage in Mice Lacking the Metabotropic Glutamate Receptor 1. Am. J. Pathol. 178, 1257-1269 (2011).

Species mouse

Application WB,IHC (frozen)

Publication Meyer-Schwesinger, C. et al. Nephrotic Syndrome and Subepithelial Deposits in a Mouse Model of Immune-Mediated Anti-Podocyte Glomerulonephritis. J. Immunol. 187, 3218-3229 (2011).

Species mouse

Application IHC (paraffin)

Publication Gödel, M. et al. Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J. Clin. Invest. 121, 2197-2209 (2011).

Species human

Application IHC (frozen)

Publication Wagner, N. et al. The podocyte protein nephrin is required for cardiac vessel formation. Hum. Mol. Genet. 20, 2182-2194 (2011).

Species mouse

Application IHC (frozen)

Publication Matsusaka, T. et al. ARB Protection Against Podocyte-Induced Sclerosis is Podocyte AT1-Independent. Hypertension 55, 967-973 (2010).

Species mouse

Application IHC

Publication Krall, P. et al. Podocyte-Specific Overexpression of Wild Type or Mutant Trpc6 in Mice Is Sufficient to Cause Glomerular Disease. PLoS One 5, (2010).

Species mouse

Application IHC (frozen)

Publication Xavier, S. et al. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS One 5, (2010).

Species human

Application IHC-IF

Publication Turk, T. et al. BMP Signaling and Podocyte Markers Are Decreased in Human Diabetic Nephropathy in Association With CTGF Overexpression. J. Histochem. Cytochem. 57, 623-631 (2009).

Species human,mouse

Application IHC (paraffin)

Publication Hirose, T. et al. An essential role of the universal polarity protein, aPKCλ, on the maintenance of podocyte slit diaphragms. PLoS One 4, (2009).

Species mouse

Application IHC

Publication Collino, F. et al. Preeclamptic sera induce nephrin shedding from podocytes through endothelin-1 release by endothelial glomerular cells. Am. J. Physiol. - Ren. Physiol. 294, 1185-1194 (2008).

Species human

Application ICC-IF

Publication Koop, K. et al. Selective Loss of Podoplanin Protein Expression Accompanies Proteinuria and Precedes Alterations in Podocyte Morphology in a Spontaneous Proteinuric Rat Model. Am. J. Pathol. 173, 315-326 (2008).

Species rat

Application IHC (paraffin)

Publication Walter, B., Schlechter, T., Hergt, M., Berger, I. & Hofmann, I. Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney. Histochem. Cell Biol. 130, 943-956 (2008).

Species human

Application IHC (frozen)

Publication Wagner, K.-D. et al. An Inducible Mouse Model for PAX2-Dependent Glomerular Disease: Insights into a Complex Pathogenesis. Curr. Biol. 16, 793-800 (2006).

Species human

Application IHC

Publication Schiwek, D. et al. Stable expression of nephrin and localization to cell-cell contacts in novel murine podocyte cell lines. Kidney Int. 66, 91-101 (2004).

Species mouse

Application ICC-IF

Publication Peitsch, W. K. Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli. J. Am. Soc. Nephrol. 14, 1452-1463 (2003).

Species human,mouse,rat,bovine

Application IHC (frozen),IEM

Publication Patek, C. E. et al. Murine Denys-Drash syndrome: evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis. Hum. Mol. Genet. 12, 2379-2394 (2003).

Species mouse

Application IHC (paraffin)

Publication Barisoni, L., Kriz, W., Mundel, P. & D‘Agati, V. The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy. J. Am. Soc. Nephrol. 10, 51-61 (1999).

Species human

Application IHC (paraffin)

Publication Kihara, I. et al. Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy. Histopathology 34, 537-47 (1999).

Species human

Application IHC (paraffin)

Publication Mundel, P., Reiser, J. & Kriz, W. Induction of Differentiation in Cultured Rat and Human Podocytes. J. Am. Soc. Nephrol. 697-705 (1997).

Species human,mouse

Application ICC-IF

Publication Mundel, P. et al. Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines. Exp. Cell Res. 236, 248-58 (1997).

Species mouse

Application ICC-IF

Publication Mundel, P. et al. Synaptopodin: An Actin-associated Protein in Telencephalic Dendrites and Renal Podocytes. J. Cell Biol. 139, 193-204 (1997).

Species rat

Application WB,IHC (frozen),IEM

Publication Mundel, P., Gilbert, P. & Kriz2, W. Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 KD Protein-. J. Histochem. Cytochem. 39, 1047-1056 (1991).

Species human,rat

Application IHC (frozen),IEM

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File IHC mouse antibody

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Datasheet

MSDS

FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.