Reagents and instruments for immunology, cell biology and molecular biology.
 
> LB Agar Miller, Animal Free (Powder) (Miller's LB agar, Luria-Bertani agar)

LB Agar Miller, Animal Free (Powder) (Miller's LB agar, Luria-Bertani agar)

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Cat# L1515-01-500g

Size : 500g

Brand : US Biological

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L1515-01 LB Agar Miller, Animal Free (Powder) (Miller's LB agar, Luria-Bertani agar)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
RT/4°C

Soy Peptone is substituted for Tryptone.||LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950’s (1,2,3,4). These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (1,5). The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin™.||BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.||Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.||Homogeneous: Proprietary blending technology ensures complete mixing of components.||Appearance: |Light tan, homogeneous, free flowing powder||Solubility: |Light tan, clear, complete after autoclaving||pH (after autoclaving):|As Reported||Companion Products:|Terrific Broth is a richer media that would be suitable if higher cell densities and higher yields for plasmids or protein purifications are necessary.|T2810: Terrific Broth (Powder)|T2810-05: Terrific Broth, Complete with Carbon Source (Powder)|T2810-10: Terrific Broth, Modified for Genomics (Powder)|T2810-11: Terrific Broth, Modified for Fermentation, non-animal (Powder)|L1500: LB Agar Lennox (Powder)|L1505: LB Broth Lennox (Powder)|L1510: LB Top Agar Lennox (Powder)|P9102-40H: Protein Expression Media, LB Broth Lennox (Powder) pEX™|P9102-40H1: Protein Expression Media, LB Broth Lennox (Liquid) pEX™|L1515: LB Agar Miller (Powder)|L1520: LB Broth Miller (Powder)|L1525: LB Top Agar Miller (Powder)|P9102-40G: Protein Expression Media, LB Broth Miller (Powder) pEX™|P9102-40G1: Protein Expression Media, LB Broth Miller (Liquid) pEX™|L1530: L-Broth (Luria Broth) (PowdBiology).

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Miller, J.H.: Experiments in molecular genetics (1972). Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 2. Luria, S.E., Burrous, J.W.: Hybridization between Escherichia coli and Shigella. J. Bacteriol. 74: 461-476 (1955). 3. Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1: 190-206 (1955). 4. Luria, S.E., Adams, J.N., Ting, R.C.: Transduction of lactose-utilizing ability among strain of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12: 348-390 (1960). 5. Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular cloning: a laboratory manual, 2nd edition (1989). Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

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