Reagents and instruments for immunology, cell biology and molecular biology.

Lactate Dehydrogenase (LDH) Activity Assay Kit

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Cat# E-BC-K046-S-100

Size : 100Assays

Brand : Elabscience

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Detection Principle	Coenzyme I as a hydrogen carrier, LDH catalyzes the production of pyruvic acid from lactic acid, pyruvic acid react with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone which is brownish red in alkaline solution. The depth of the color is proportional to the concentration of pyruvic acid. The activity of LDH can be measured indirectly by measuring the OD value at 450 nm.
Synonyms	LDH
Sample Type	Serum,Plasma,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (450 nm)
Research Area	Glycolysis And Lipid Metabolism , Liver And Renal Function , Energy Metabolism
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	4 U/L
Detection Range	4-400 U/L
Precision	inter-assay CV: 7.2% \| intra-assay CV: 2.2%
Sample Volume	400 μL
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-K008-M	Monoamine Oxidase (MAO) Activity Assay Kit	96T , 48T
E-BC-K235-M	Alanine Aminotransferase (ALT/GPT) Activity Assay Kit	96T , 48T
E-BC-K236-M	Aspartate Aminotransferase (AST/GOT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	10-15
Human plasma	10-15
Porcine serum	20-30
Rat serum	20-30
Rat plasma	20-30
10% Rat liver tissue homogenate	800-1000
10% Mouse kidney tissue homogenate	500-800
10% Rat lung tissue homogenate	300-500

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.