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KRT14, 1-472aa, Human,His tag, E.coli

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Cat# 01-P1225-500ug

Size : 500ug

Brand : American Research Products

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KRT14, 1-472aa, Human

Category: Proteins

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01-P1225

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Product Name	KRT14, 1-472aa, Human
Description	KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms	Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI
Host	E. coli
Molecular Weight	24.9kDa (227aa), confirmed by MALDI-TOF
Amino Acid Sequence	MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN
Tag	His-tag
Reactivity	Human
Applications	SDS-PAGE
Form	Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl, 1mM EDTA
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 90% by SDS-PAGE
Storage	Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles.
References	Romano RA. et al. (2007) J Invest Dermatol. 127:1175-1186. Coulombe PA. et al. (1991) Cell. 66 (6): 1301-11.
Background	KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Supplier	ARP

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