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Ionomycin calcium salt is a calcium ionophore [1].
Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.
Ionomycin calcium salt is a calcium ionophore. In cultured chicken pectoralis muscle, ionomycin (10-6-10-5 M) selectively increased the incorporation of methionine into two proteins. Ionomycin increased influx of extracellular Ca2+ and increased Ca2+ concentration in the cytoplasm, which then regulated the synthesis of muscle proteins [2]. In rat parotid gland, ionomycin increased 86Rb efflux, 22Na uptake and 3H-protein secretion, which were dependent on increasing cytosolic Ca2+. In parotid cells, ionomycin released the receptor-regulated cellular Ca2+ pool [3]. In human bladder cancer cells HT1376, ionomycin significantly inhibited cell growth in a time- and dose-dependent way and induced apoptotic DNA degradation. Also, ionomycin reduced the ratios of Bcl-2 to Bax mRNA and protein [4].
In athymic nude mice bearing HT1376 tumors, ionomycin significantly inhibited tumor growth and reduced the tumorigenicity [4].
References:[1]. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore. J Biol Chem, 1978, 253(17): 5892-5894.[2]. Roufa D, Wu FS, Martonosi AN. The effect of Ca2+ ionophores upon the synthesis of proteins in cultured skeletal muscle. Biochim Biophys Acta, 1981, 674(2): 225-237. [3]. Poggioli J, Leslie BA, McKinney JS, et al. Actions of ionomycin in rat parotid gland. J Pharmacol Exp Ther, 1982, 221(1): 247-253.[4]. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. J Urol, 1999, 162(3 Pt 1): 916-921.
Cell lines
Human bladder cancer cell line HT1376
Reaction Conditions
0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation
Applications
The in vitro growth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells.
Animal models
Athymic nude mice bearing HT1376 tumors
Dosage form
50 μg of ionomycin in 100 μl PBS
Injected intratumorally three times weekly for 4 weeks
Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, these in vivo growth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin.
Note
The technical data provided above is for reference only.
References:
1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921.
