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> IgG2a (TRITC)

IgG2a (TRITC)

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Cat# I1904-83S-1mg

Size : 1mg

Brand : US Biological


I1904-83S Rabbit Anti-IgG2a (TRITC)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications: |Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.||Recommended Dilution: |Optimal dilutions to be determined by the researcher. ||Label:|Tetramethylrhodamine isothiocyanante (TRITC) (MW 444D)||Absorption Wavelength:|550 nm ||Emission Wavelength: |570 nm||Fluorochrome/Protein Ratio: |2.1 moles TRITC per mole of Rabbit IgG||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. TRITC conjugates are sensitive to light.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (after reconstitution) (UV280)|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with1ml sterile dH2O. Labeled with tetramethylrhodamine isothiocyanante (TRITC).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgG2a heavy chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, mouse IgG and mouse serum.| < 1% Crossreactivity against other mouse or human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgG2a heavy chain
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with1ml sterile dH2O. Labeled with tetramethylrhodamine isothiocyanante (TRITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, mouse IgG and mouse serum.| < 1% Crossreactivity against other mouse or human heavy or light chain isotypes.