Reagents and instruments for immunology, cell biology and molecular biology.

IgG, IgA, IgM, H&L, X-adsorbed (TRITC)

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Cat# I1904-35M-1mg

Size : 1mg

Brand : US Biological

I1904-35M Rabbit Anti-IgG, IgA, IgM, H&L, X-adsorbed (TRITC)

Clone Type

Polyclonal

Host

goat

Source

mouse

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

E IF WB

Crossreactivity

Gt Mo

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.||Recommended Dilution: |Optimal dilutions to be determined by the researcher.||Label:|Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons)||Absorption Wavelength: 550nm ||Emission Wavelength: 570nm||Fluorochrome/Protein Ratio: 3.6 moles TRITC per mole of Goat IgG F(ab’)2||Storage and Stability:|Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C.|Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: TRITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: mouse|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide. Labeled with TRITC.|Purity: Purified by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |Immunogen: Mouse IgG, IgA, and IgM whole molecules|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Mouse IgG, IgA, and IgM whole molecules

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide. Labeled with TRITC.

Purity

Purified by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.