IFNG, Recombinant, Human, aa24-161, His-Tag (Interferon Gamma, IFG, IFI, Interferon Gamma)

IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143aa residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-Tag at N-terminal, expressed in E.coli and purified by using conventional chromatography techniques.||Source:|Recombinant protein corresponding to aa24-161 of human IFNG protein, fused to His-Tag at N-terminal, expressed in E. coli.||Molecular Weight: |~18.4kD (159aa), Confirmed by MALDI-TOF||Endotoxin: |<1EU/1ug (determined by LAL method)||Amino Acid Sequence:|MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG||Biological Activity:|Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 5ng/ml.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.