- More Files
Immunocytochemistry
Immunocytochemical staining of HeLa cells with HSP90AA1/HSP90AB1 monoclonal antibody, clone Hyb-K41220A (ATTO 488) (Cat # MAB18059). (A) DAPI (blue) nuclear stain. (B) Anti-HSP90AA1/HSP90AB1 Antibody. (C) Composite.
- Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant human HSP90AA1/HSP90AB1.
Immunogen
Recombinant protein corresponding to amino acids 291-304 of human HSP90 alpha.
Host
Mouse
Reactivity
Human
Form
Liquid
Conjugation
ATTO 488
Purification
Protein G purification
Isotype
IgG2a
Recommend Usage
ELISA
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunocytochemical staining of HeLa cells with HSP90AA1/HSP90AB1 monoclonal antibody, clone Hyb-K41220A (ATTO 488) (Cat # MAB18059). (A) DAPI (blue) nuclear stain. (B) Anti-HSP90AA1/HSP90AB1 Antibody. (C) Composite.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
- Gene Info — HSP90AA1
Entrez GeneID
3320Protein Accession#
P07900;P08238Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
- Gene Info — HSP90AB1
Entrez GeneID
3326Protein Accession#
P07900;P08238Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
- Interactomes
- Pathways
- Diseases