HRP Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed
Cat# 20470-1mg
Size : 1mg
Brand : Biotium
Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed
Highly cross-adsorbed goat anti-human IgG (H L) secondary antibody labeled with our superior CF® Dyes.
Technology
CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings
Tech Tips
Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Product Description
This is a highly cross-adsorbed goat anti-human IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, and mouse serum.
- Highly cross-adsorbed for specific staining with minimal background
- Available in 11 bright and photostable CF® Dyes
- HRP and R-PE conjugates also available
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20022-1 | CF®488A Features |
| 0.5 mL (1 mg) | 20022 | |||
| 1 mg | 20022-1mg | |||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20319-1 | CF®543 Features |
| 0.5 mL (1 mg) | 20319 | |||
| 1 mg | 20319-1mg | |||
| CF®555 | 555/565 nm | 50 uL (100 ug) | 20230-1 | CF®555 Features |
| 0.5 mL (1 mg) | 20230 | |||
| 1 mg | 20230-1mg | |||
| CF®568 | 562/583 nm | 50 uL (100 ug) | 20097-1 | CF®568 Features |
| 0.5 mL (1 mg) | 20097 | |||
| 1 mg | 20097-1mg | |||
| CF®583R | 585/609 nm | 50 uL (100 ug) | 20901-50uL | CF®583R Features |
| 0.5 mL (1 mg) | 20901-500uL | |||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20154-1 | CF®594 Features |
| 0.5 mL (1 mg) | 20154 | |||
| 1 mg | 20154-1mg | |||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20132-1 | CF®633 Features |
| 0.5 mL (1 mg) | 20132 | |||
| 1 mg | 20132-1mg | |||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20081-1 | CF®640R Features |
| 0.5 mL (1 mg) | 20081 | |||
| 1 mg | 20081-1mg | |||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20280-1 | CF®647 Features |
| 0.5 mL (1 mg) | 20280 | |||
| 1 mg | 20280-1mg | |||
| CF®680 | 681/698 nm | 50 uL (100 ug) | 20287-1 | CF®680 Features |
| 0.25 mL (500 ug) | 20287 | |||
| CF®770 | 770/797 nm | 50 uL (100 ug) | 20288-1 | CF®770 Features |
| 0.25 mL (500 ug) | 20288 | |||
| R-PE | 496, 546, 565/578 nm | 200 uL (100 ug) | 20355-200uL | |
| 1 mL (500 ug) | 20355-1mL | |||
| HRP | N/A | 100 uL (100 ug) | 20470-100uL | |
| 1 mL (1 mg) | 20470-1mL | |||
| 1 mg | 20470-1mg |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
CF is a registered trademark of Biotium, Inc.
References
Download a list of curated CF® Dye references.