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- Specifications
Product Description
Mouse monoclonal antibody raised against native HLA-Class I.
Immunogen
Native purified HLA-Class I from membrane of human tonsil cells.
Host
Mouse
Reactivity
Bovine, Cat, Human, Primates
Specificity
This antibody recognizes MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
Form
Liquid
Conjugation
FITC
Concentration
1 mg/mL
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1:300)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Flow Cytometry
- Publication Reference
- Expression of HLA-G in human cornea, an immune-privileged tissue.
Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED.
Human Immunology 2003 Nov; 64(11):1039.
- The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.
Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V.
Immunogenetics 2001 Aug; 53(6):440.
- Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody.
J J Ladasky, B P Shum, F Canavez, H N Seuanez, P Parham.
Immunogenetics 1999 Apr; 49(4):312.
Application：Flow Cyt, Monkey, OM VI-3 cells, Owl monkey 531H cells.
- Expression of HLA-G in human cornea, an immune-privileged tissue.