Non-diluted antibody is stable for 4 years at 2-8 % to prevent loss of enzymatic activity. Such solution will not freeze in -20:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Human IgG Fc (two heavy chains with constant domains)
Predicted reactivity:
Human IgG Fc (two Heavy chains with constant domains)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Additional Information
Additional information:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Additional information (application):
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.
No reactivity is observed with the light chains on human immunoglobulins and to human IgA or IgM based on immunoelectrophoresis.
Background
Background:
Goat anti-human IgG Fc (two heavy chains with constant domains)is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
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