Fibronectin (8th Type III Repeat)(568), CF568 conjugate, 0.1mg/mL
Cat# BNC680351-500
Size : 500uL
Brand : Biotium
| Antibody number | #0351 |
|---|---|
| Antibody reactivity (target) | Fibronectin |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | 568 |
| Isotype | IgG1, kappa |
| Molecular weight | 220 kDa (monomer); 440 kDa (dimer) |
| Synonyms | Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3 |
| Human gene symbol | FN1 |
| Entrez gene ID | 2335 |
| SwissProt | P02751 |
| Unigene | 203717 |
| Immunogen | High molecular weight proteins secreted by cultivated human fibroblasts |
| Verified antibody applications | IHC (FFPE) (verified) |
| Antibody target cellular localization | Extracellular matrix |
| Species reactivity | Human |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT, Flow cytometry: 0.5-1 ug/million cells, Immunofluorescence: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user |
| Positive control | SW156 cells. Kidney. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Antibody research areas | Cell adhesion, Extracellular matrix |
Product Description
The epitope of this antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues. Catalog number key for antibody number 0351, Anti-Fibronectin (568)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |