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For an Accurate & Robust PCR Amplification of Fragments up to 15 kb
FastPANGEA kb due to its inability to repair nucleotide mismatches following misincorporation. The addition of a small quantity of proofreading enzyme allows mismatches to be repaired and extension to continue, resulting in the amplification of long amplicons with high yield. The presence of the proofreading polymerase significantly increases fidelity (6.5x) as compared to Taq DNA Polymerase alone. This mixture of enzymes allows for long and accurate PCR amplification of targets from a variety of templates, such as 5-15 kb of genomic DNA.
It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. As well, it possesses 3_tb_body_wrapper et_pb_module "> 5 U/ U
Detailed information:
Unit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 mM TAPS-HCl, pH 9.0 (at 25 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
– 100 U FastPANGEA; 500 μL Buffer PA (5x) – 250 μL MgCl2 (50 mM) – 60 μL DMSO (100%)
This product is developed, designed and sold_tb_body et_pb_toggle_close">
