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Immunofluorescence
Application

Immunofluorescence

Immunofluorescenct staining of Jurkat cells with CD81 recombinant monoclonal antibody, clone 1D6 (Cat # RAB03346).
Immunofluorescence analysis of paraformaldehyde Jurkat cells fixed on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of RAB03346 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

  • Specifications

    Product Description

    Mouse recombinant monoclonal antibody raised against human CD81.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Mouse

    Immunogen

    Original antibody is raised against 5A6-aggregated OCI-LY8 B cells.

    Reactivity

    Chimpanzee, Goat, Human, Sheep

    Form

    Liquid

    Conjugation

    Unconjugated

    Concentration

    batch dependent

    Isotype

    IgG1, kappa

    Recommend Usage

    ELISA
    Flow Cytometry
    Functional Study
    Immunocytochemistry
    Immunofluorescence
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunoprecipitation
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS with 0.02% Proclin 300

    Storage Instruction

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunofluorescence

    Immunofluorescenct staining of Jurkat cells with CD81 recombinant monoclonal antibody, clone 1D6 (Cat # RAB03346).
    Immunofluorescence analysis of paraformaldehyde Jurkat cells fixed on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of RAB03346 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    Functional Study

    Flow Cytometry

  • Gene Info — CD81

    Entrez GeneID

    975

    Gene Name

    CD81

    Gene Alias

    S5.7, TAPA1, TSPAN28

    Gene Description

    CD81 molecule

    Omim ID

    186845

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq

    Other Designations

    26 kDa cell surface protein TAPA-1|CD81 antigen|CD81 antigen (target of antiproliferative antibody 1)|target of antiproliferative antibody 1

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