CD19 Polyclonal Antibody
Cat# bs-0079R
Size : 100ul
Brand : Bioss
CD19 Polyclonal Antibody
Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-CD19 Polyclonal Antibody (bs-0079R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysate probed with Rabbit Anti-CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 59kD. Observed band size: 28kD and 90kD
Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human Raji cells probed with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0079R |
| Product Name | CD19 Polyclonal Antibody |
| Applications | ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Reactivity | Human, Mouse, Rat, Sheep |
| Predicted Reactivity | Cow, Pig, Horse, Guinea Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat CD19 |
| Immunogen Range | 485-556/556 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 12478 |
| Swiss Prot | F1LNH2 |
| Subcellular location | Cell membrane |
| Synonyms | AW495831; B-lymphocyte antigen CD19; Differentiation antigen CD19; CD19 |
| Background | Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
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