CD134 Ligand CD8a, Fusion Protein

Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells.  CD134L binds to CD134 present on  activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of  immflamation and rejection (5,6,7,8).||Functional Application: CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.||Human CD134L-muCD8 Fusion Protein*||Molecular Structure: A soluble molecule consisting of the extracellular domain (132 aa) of human CD134L fused to the extracellular domain (167aa) of murine CD8 alpha.||Transfectant Cell Line: CHO||Applications: |Suitable for use in ELISA, Flow cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: protein binds to CD134 positive cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.|