Reagents and instruments for immunology, cell biology and molecular biology.
 
> Catalase (CAT) Activity Fluorometric Assay Kit

Catalase (CAT) Activity Fluorometric Assay Kit

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Cat# E-BC-F006-500

Size : 500Assays

Brand : Elabscience

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Detection Principle Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
Synonyms CAT
Sample Type Serum,Plasma,Animal tissue
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Oxidative Stress ,  Plant Physiology Series
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.01 U/L
Detection Range 0.01-6.51 U/L
Precision inter-assay CV: 6.8% | intra-assay CV: 3.4%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K031-M Catalase (CAT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Mouse serum 60-80
Mouse plasma 60-80
Rat serum 70-80
Human saliva 30-50
Rat urine 30-50
10% Mouse liver tissue homogenate 2500-3000
10% Mouse lung tissue homogenate 200-400
10% Rat muscle tissue homogenate 100-200
10% Rat brain tissue homogenate 40-50
10% Mouse kidney tissue homogenate 2000-2500

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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