Canine IL-1β ELISA Data

Canine IL-1β ELISA Kit Components

Canine IL-1β ELISA Specifications

The Canine IL-1β Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Canine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately. 

The Canine IL-1β Do-It-Yourself ELISA can also be used to measure Dingo IL-1β.

For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.

IL-1β Background

The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.

Alternate Names - IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta