Canine IFN-γ>

Canine IFN-γ>

Component	Usage	Quantity	Catalog #
Anti-Canine IFNγ Polyclonal Antibody	Capture Antibody	100 µg	PB0386D-100
Biotinylated Anti-Canine IFNγ Polyclonal Antibody	Detection Antibody	50 µg	PBB0394D-050
Canine IFNγ Recombinant Protein	Standard	5 µg	RP0271D-005

 

Specifications

The Canine IFNγ Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Canine IFNγ ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately. 

The Canine IFNγ Do-It-Yourself ELISA can also be used to measure Dingo, Red Fox, Raccoon Dog, and Harbor Seal IFNγ.

For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.

Background

Interferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Alternate Names - IFNG, IFG, IFI, interferon, gamma, interferon gamma