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> beta Tubulin Monoclonal Antibody

beta Tubulin Monoclonal Antibody

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Cat# E-AB-20093-120

Size : 120uL

Brand : Elabscience

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Verified Samples Verified Samples in WB: Hela, Mouse brain, Rat brain
Verified Samples in IF: Hela
Dilution WB 1:5000-10000,  IF 1:100-200
Isotype IgG
Host Mouse
Reactivity Human,  Mouse,  Rat
Applications WB,  IF
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre β-tubulin
Synonyms CDCBM,  CDCBM1,  CFEOM3,  CFEOM3A,  FEOM3,  M(beta)3,  M(beta)6,  MC1R,  Neuron specific beta III Tubulin,  Neuron-specific class III beta-tubulin,  QccE-11995,  QccE-15186,  TBB3,  TUBB3,  TUBB4,  Tubb 3,  Tubulin,  Tubulin beta 3,  Tubulin beta 3 chain,  Tubulin beta 4,  beta 3 tubulin,  beta-4
Swissprot
Calculated MW 50 kDa
Observed MW 55 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Cytoplasm>cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Tags and Cell Markers
Clone No. 4A6
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background TUBB3,the class III β tubulin or Tuj1,is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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Description
Cond.
Price Bef. VAT
E-AB-20093-200
beta Tubulin Monoclonal Antibody
 200uL