Reagents and instruments for immunology, cell biology and molecular biology.
 
> BCIP [6578-06-9]

BCIP [6578-06-9]

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Cat# HY-15909-100mg

Size : 100mg

Brand : MedChemExpress

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Description

BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity[1].

In Vitro

BCIP produces the blue-color enzymatic reaction product BCI during hydrolysis by ALP in HepG2 cell, which has strong SERRS activity and can be used for ALP activity detection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BCIP Related Antibodies

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (115.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 25 mg/mL (57.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3062 mL 11.5308 mL 23.0617 mL
5 mM 0.4612 mL 2.3062 mL 4.6123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

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