Ascorbic Acid Assay Kit, BioAssay™

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. This Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample. This kit is suitable for the determination of ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates.||Sample size: 20ul ||Linear Detection Range: |Colorimetric Assay: 6 to 1000uM ascorbic acid|Fluorometric Assay: 1 to 100uM ascorbic acid||Applications:|Assays: Ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates|Drug Discovery/Pharmacology: Effects of drugs on ascorbic acid metabolism||Kit Components:|A3774-61A: Enzyme Mix, 1x120ul|A3774-61B: Standard, 1x400ul. 10mM Ascorbic acid|A3774-61C: Assay Buffer, 1x10ml|A3774-61D: Dye Reagent, 1x120ul||Materials Required But Not Provided:|Optical density plate reader|Fluorescence plate reader.|Pipeting devices|Centrifuge tubes|Clear flat-bottom uncoated 96-well plates|Black flat-bottom uncoated 96-well plates||Storage and Stability:|Store all kit components at --20°C. Kit is stable for 6 months.