Reagents and instruments for immunology, cell biology and molecular biology.

Anti-WNT10B antibody (260-340)

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Cat# NB-22-50216-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Immunofluorescence analysis of U-2 OS cells using WNT10B Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using WNT10B Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using WNT10B Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of WNT10B in paraffin-embedded human colon carcinoma using WNT10B Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of WNT10B in paraffin-embedded rat ovary using WNT10B Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of lysates from HeLa cells, using WNT10B Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-WNT10B (260-340) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	WNT10B
Gene ID:	7480
Uniprot ID:	WN10B_HUMAN
Immunogen Region:	260-340
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-340 of human WNT10B (NP_003385.2).
Immunogen Sequence:	TCWRAAPEFRAVGAALRERL GRAIFIDTHNRNSGAFQPRL RPRRLSGELVYFEKSPDFCE RDPTMGSPGTRGRACNKTSR L

Description

Tissue Specificity	Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
Post Translational Modifications	Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function	Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Protein Name	Protein Wnt-10b Protein Wnt-12
Database Links	Reactome: R-HSA-3238698 Reactome: R-HSA-373080 Reactome: R-HSA-381340
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Protein Wnt-10b antibody Anti-Protein Wnt-12 antibody Anti-WNT10B antibody Anti-WNT12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance