Reagents and instruments for immunology, cell biology and molecular biology.

Anti-WNT1 antibody (301-350 aa)

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Cat# NB-22-6270-50

Size : 50ug

Brand : Neo Biotech

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Western blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Proto-oncogene Wnt-1 (301-350 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	WNT1
Gene ID:	7471
Uniprot ID:	WNT1_HUMAN
Immunogen Region:	301-350 aa
Specificity:	Wnt-1 Polyclonal Antibody detects endogenous levels of Wnt-1 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human WNT1 at the amino acid range 301-350

Description

Function	Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.
Protein Name	Proto-Oncogene Wnt-1 Proto-Oncogene Int-1 Homolog
Database Links	Reactome: R-HSA-201681 Reactome: R-HSA-3238698 Reactome: R-HSA-373080 Reactome: R-HSA-381340 Reactome: R-HSA-4086400 Reactome: R-HSA-4641262
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Proto-Oncogene Wnt-1 antibody Anti-Proto-Oncogene Int-1 Homolog antibody Anti-WNT1 antibody Anti-INT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance