Anti-SGK1 antibody (1-100) (Clone: S9MR)

Clonality:	Monoclonal
Clone ID:	S9MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	SGK1
Gene ID:	6446
Uniprot ID:	SGK1_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SGK1 (O00141).
Immunogen Sequence:	MTVKTEAAKGTLTYSRMRGM VAILIAFMKQRRMGLNDFIQ KIANNSYACKHPEVQSILKI SQPQEPELMNANPSPPPSPS QQINLGPSSNPHAKPSDFHF

Tissue Specificity	Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
Post Translational Modifications	Regulated by phosphorylation. Activated by phosphorylation on Ser-422 by mTORC2, transforming it into a substrate for PDPK1 which phosphorylates it on Thr-256. Phosphorylation on Ser-397 and Ser-401 are also essential for its activity. Phosphorylation on Ser-78 by MAPK7 is required for growth factor-induced cell cycle progression. Ubiquitinated by NEDD4L.which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum.which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability.
Function	Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na(+) retention, renal K(+) elimination, salt appetite, gastric acid secretion, intestinal Na(+)/H(+) exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na(+) channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K(+) channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na(+)/K(+) ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na(+) transport than isoform 1.
Protein Name	Serine/Threonine-Protein Kinase Sgk1 Serum/Glucocorticoid-Regulated Kinase 1
Database Links	Reactome: R-HSA-1257604 Reactome: R-HSA-2672351 Reactome: R-HSA-6804757 Reactome: R-HSA-8986944 Reactome: R-HSA-9031628
Cellular Localisation	Cytoplasm Nucleus Endoplasmic Reticulum Membrane Cell Membrane Mitochondrion The Subcellular Localization Is Controlled By The Cell Cycle As Well As By Exposure To Specific Hormones And Environmental Stress Stimuli In Proliferating Cells It Shuttles Between The Nucleus And Cytoplasm In Synchrony With The Cell Cycle And In Serum/Growth Factor-Stimulated Cells It Resides In The Nucleus In Contrast After Exposure To Environmental Stress Or Treatment With Glucocorticoids It Is Detected In The Cytoplasm And With Certain Stress Conditions Is Associated With The Mitochondria In Osmoregulation Through The Epithelial Sodium Channel It Can Be Localized To The Cytoplasmic Surface Of The Cell Membrane Nuclear Upon Phosphorylation Isoform 2: Cell Membrane
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase Sgk1 antibody Anti-Serum/Glucocorticoid-Regulated Kinase 1 antibody Anti-SGK1 antibody Anti-SGK antibody

Information sourced from Uniprot.org

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