Anti-Mouse CD11a (LFA-1α) (Clone I21/7) - FITC
Cat# C225-500
Size : 500µg
Brand : Leinco Technologies
AntiMouse CD11a (LFA1α) (Clone I21/7) – FITC
AntiMouse CD11a (LFA1α) (Clone I21/7) – FITC
Product No.: C225
Clone I21/7 Target CD11a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LFA1α Isotype IgG2a Applications FC |
Data
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This Fluorescein conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient Excitation Laser Blue Laser (490 nm) RRIDAB_2829337 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this I21/7 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone I21/7 recognizes an epitope on mouse CD11a. Background LFA1α (CD11a) and CD18 are the Integrin alphaL and beta2 chains respectively that combine to form LFA1, a glycoprotein and a member of the Integrin family. Integrin alphaL/beta2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA1 participates in the immunological synapses between CD8+ T lymphocytes and antigenpresenting cells. The absence of LFA1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyteendothelial cell interaction, cytotoxic Tcell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophagelike cell lines. Ligand/Receptor ICAM1 (CD54), ICAM2(CD102), ICAM3(CD50) PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Signal Transduction References & CitationsWuthrich, R. P. et al. (1991) Cell Immunol. 144:22 |