Anti-Human IgE APC MAb(Clone :4H10)
Fig 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/ml).
Anti-Human IgE APC MAb(Clone :4H10)
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | 0.1 mg/ml Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Immunogen Information : | Purified human IgE. |
Specificity: The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Flow cytometry: Recommended dilution: 0.5-3 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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