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> Anti-HK1 antibody (Clone: 3A10)

Anti-HK1 antibody (Clone: 3A10)

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Cat# NB-22-8249-200

Size : 200ug

Brand : Neo Biotech

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  • Flow cytometric analysis of K562 cells using HXK I monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded kidney tissues with DAB staining using HXK I monoclonal antibody.
  • Immunofluorescence analysis of NIH/3T3 cells using HXK I monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using HXK I monoclonal antibody against Jurkat (1) , HeLa (2) , HepG2 (3) and NIH/3T3 (4) cell lysate.

General Info

Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Hexokinase-1 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 3A10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: HK1
Gene ID: 3098
Uniprot ID: HXK1_HUMAN
Specificity: HXK I Monoclonal Antibody detects endogenous levels of HXK I protein.
Immunogen: Purified recombinant fragment of human HXK I expressed in E. Coli.

Description

Function Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Protein Name Hexokinase-1
Brain Form Hexokinase
Hexokinase Type I
Hk I
Hexokinase-A
Database Links Reactome: R-HSA-446205
Reactome: R-HSA-5619056
Reactome: R-HSA-70171
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
The Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)
Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-Glucosamine
Leading To Relocation To The Cytosol
Alternative Antibody Names Anti-Hexokinase-1 antibody
Anti-Brain Form Hexokinase antibody
Anti-Hexokinase Type I antibody
Anti-Hk I antibody
Anti-Hexokinase-A antibody
Anti-HK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance