Reagents and instruments for immunology, cell biology and molecular biology.

Anti-FDFT1 antibody (1-260)

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Cat# NB-22-22516-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Immunofluorescence analysis of NIH-3T3 cells using SQS/FDFT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse brain using SQS/FDFT1 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using SQS/FDFT1 antibody at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of extracts of various cell lines, using SQS/FDFT1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-SQS/FDFT1 (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	FDFT1
Gene ID:	2222
Uniprot ID:	FDFT_HUMAN
Immunogen Region:	1-260
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SQS/FDFT1 (NP_004453.3).
Immunogen Sequence:	MEFVKCLGHPEEFYNLVRFR IGGKRKVMPKMDQDSLSSSL KTCYKYLNQTSRSFAAVIQA LDGEMRNAVCIFYLVLRALD TLEDDMTISVEKKVPLLHNF HSFLYQPDWRFMESKEKDRQ VLEDFPTISLEFRNLAEKYQ TVIADICRRMGIGMAEFLDK HVTSEQEWDKYCHYVAGLVG IGLSRLFSASEFEDPLVGED TERANSMGLFLQKTNIIRDY LEDQQGGREFWPQEVWSRY

Description

Tissue Specificity	Widely expressed.
Function	Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
Protein Name	Squalene Synthase Sqs Ss Fpp -Fpp Farnesyltransferase Farnesyl-Diphosphate Farnesyltransferase Farnesyl-Diphosphate Farnesyltransferase 1
Database Links	Reactome: R-HSA-191273 Reactome: R-HSA-1989781 Reactome: R-HSA-2426168
Cellular Localisation	Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein
Alternative Antibody Names	Anti-Squalene Synthase antibody Anti-Sqs antibody Anti-Ss antibody Anti-Fpp -Fpp Farnesyltransferase antibody Anti-Farnesyl-Diphosphate Farnesyltransferase antibody Anti-Farnesyl-Diphosphate Farnesyltransferase 1 antibody Anti-FDFT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance