Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Doublecortex/DCX antibody (Internal)

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Cat# NB-22-60442-100

Size : 100ug

Brand : Neo Biotech

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STJ73591 (0. 003µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ73591 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

General Info

Host:	Goat
Applications:	Pep-ELISA/WB/IHC/IF
Reactivity:	Human/Mouse/Rat/Dog/Cat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-Doublecortex/DCX (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	WB-0.003-0.01µg/ml IF-Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. 5µg/ml ELISA-antibody detection limit dilution 1:128000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Information

Gene Symbol:	DCX
Gene ID:	1641
Uniprot ID:	DCX_HUMAN
Immunogen Region:	Internal
Accession Number:	NP_000546.2; NP_835365.1; NP_835364.1; NP_835366.1
Specificity:	This antibody is expected to recognise isoform a (NP_000546.2) , isoform b (NP_835365.1) , and isoform c (NP_835364.1 and NP_835366.1).
Immunogen Sequence:	KASPTPQKTSAKSPG

Description

Post Translational Modifications	Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Function	Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Protein Name	Neuronal Migration Protein Doublecortin Doublin Lissencephalin-X Lis-X
Database Links	Reactome: R-HSA-447043
Cellular Localisation	Cytoplasm Cell Projection Neuron Projection Localizes At Neurite Tips
Alternative Antibody Names	Anti-Neuronal Migration Protein Doublecortin antibody Anti-Doublin antibody Anti-Lissencephalin-X antibody Anti-Lis-X antibody Anti-DCX antibody Anti-DBCN antibody Anti-LISX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance