Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CYP11A1 antibody (1-100)

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Cat# NB-22-18580-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

Immunofluorescence analysis of paraffin-embedded human placenta using CYP11A1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of CYP11A1 in paraffin-embedded human colon using CYP11A1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of lysates from HeLa cells, using CYP11A1 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western blot analysis of various lysates using CYP11A1 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western blot analysis of various lysates using CYP11A1 Rabbit polyclonal antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-CYP11A1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	CYP11A1
Gene ID:	1583
Uniprot ID:	CP11A_HUMAN
Immunogen Region:	1-100
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of CYP11A1 (NP_000772.2).
Immunogen Sequence:	MLAKGLPPRSVLVKGCQTFL SAPREGLGRLRVPTGEGAGI STRSPRPFNEIPSPGDNGWL NLYHFWRETGTHKVHLHHVQ NFQKYGPIYREKLGNVESVY

Description

Function	A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Protein Name	Cholesterol Side-Chain Cleavage Enzyme - Mitochondrial Cypxia1 Cholesterol Desmolase Cytochrome P450 11a1 Cytochrome P450(Scc
Database Links	Reactome: R-HSA-196108 Reactome: R-HSA-211976 Reactome: R-HSA-5579026
Cellular Localisation	Mitochondrion Inner Membrane Peripheral Membrane Protein Localizes To The Matrix Side Of The Mitochondrion Inner Membrane
Alternative Antibody Names	Anti-Cholesterol Side-Chain Cleavage Enzyme - Mitochondrial antibody Anti-Cypxia1 antibody Anti-Cholesterol Desmolase antibody Anti-Cytochrome P450 11a1 antibody Anti-Cytochrome P450(Scc antibody Anti-CYP11A1 antibody Anti-CYP11A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance