Reagents and instruments for immunology, cell biology and molecular biology.

Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) (AAT/1378), 0.2mg/mL

Print

Cat# BNUB1378-500

Size : 500uL

Brand : Biotium

Request more information

Alpha-1 Antitrypsin Monoclonal Mouse Antibody (AAT/1378)

Product Attributes

research-areas	Cancer, Inflammation
Antibody number	#1378
Antibody reactivity (target)	Alpha-1 Antitrypsin
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	AAT/1378
Isotype	IgG1
Molecular weight	54 kDa
Synonyms	Alpha-1 antitrypisin; SERPINA1; Alpha-1 protease inhibitor; Alpha-1 antiproteinase; AAT; Serpin A1
Human gene symbol	SERPINA1
Entrez gene ID	5265
SwissProt	P01009
Unigene	525557
Immunogen	Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)
Verified antibody applications	IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization	Secreted (extracellular)
Species reactivity	Human
Antibody application notes	Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control	HepG2 Cells. Tonsil, Hepatocellular Carcinoma or Histiocytoma.
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Cancer, Inflammation
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Tumor expression	Liver cancer

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Primary & Secondary Antibody Conjugates

Product Description

This antibody recognizes a protein of 54 kDa, which is identified as Alpha-1 antitrypsin (AAT). AAT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend-usa.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 1378, Anti-Alpha-1 Antitrypsin (AAT/1378)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.