β - galactosidase (β –GAL) Activity Assay Kit
Detection principle
β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm.
Performance characteristics
Synonyms | β-GAL |
Sample type | cells, animal and plant tissue |
Sensitivity | 16.19 U/L |
Detection range | 16.19-1200 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 70 min |
Precision | Average inter-assay CV: 6%Average intra-assay CV: 3% |
Other instruments required | Water bath, Incubator, Centrifuge, Microplate reader |
Other reagents required | 2 mmol/L hydrochloric acid |
Storage | Reagent 2: -20℃; Others: 2-8℃ |
Valid period | 12 months |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits