Rituximab (Rituxan) Immunogenicity ELISA
Cat# EL-141-181
Size : 1X96wells
Brand : AffinityImmuno
Rituximab ELISA Rituxan ELISA immunogenicity Biosimilars
INTRODUCTION
Rituximab (Rituxan® ) is a chimeric mouse/human monoclonal antibody used for treating B cell malignancy, autoimmune conditions, and graft rejection by depleting B cells from the body. This immunogenicity assay employs the bridging ELISA technique. Four levels of QC samples give a qualitative reference signal which can be used to determine the level of anti- Rituximab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
PRINCIPLE OF THE ASSAY
This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Rituximab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Rituximab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Rituxumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
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Catalog Number:
EL-141-181 -
Application(s)
measure anti-rituximab antibodies -
Target Protein
rituxan -
Alternative Name of Target
rituximab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
7.8ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Product Specifications
-
Catalog Number:
EL-141-181 -
Application(s)
measure anti-rituximab antibodies -
Target Protein
rituxan -
Alternative Name of Target
rituximab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
7.8ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3