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Rat Monoclonal Anti-CD11b / MAC-1 (Microglial Marker) [Clone M1/70]
Cat# NB-36-00375-P1ABX
Size : 100ug
Brand : Neo Biotech
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Printable Datasheet
CD11b / MAC-1 (Microglial Marker) Antibody [M1/70]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Applications & Dilutions
Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Summary
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin 2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.
Product Properties & Targets
Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Mouse, Rabbit, Rhesus Monkey
Research Areas
Cardiovascular, Immunology, Neuroscience, AKT Signaling, Complement System, Cytokine Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Membrane raft
Gene Name
ITGAM
Positive Control
Monocytes & granulocytes. Lymph nodes and tonsils.
Immunogen
B10 Mouse spleen cells enriched for T lymphocytes
Alternate Names
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11 antigen-like family member B; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; Mac-1a; MAC1; Mac1, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit
Functions
- Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Key References
- Springer T, et al. 1978. Eur. J. Immunol. 8:539.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2457584/
- https://pubmed.ncbi.nlm.nih.gov/2833753/
- https://pubmed.ncbi.nlm.nih.gov/2454931/
- https://pubmed.ncbi.nlm.nih.gov/8419480/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/2563162/
- https://pubmed.ncbi.nlm.nih.gov/1683702/
- https://pubmed.ncbi.nlm.nih.gov/1346576/
- https://pubmed.ncbi.nlm.nih.gov/3539202/
- https://pubmed.ncbi.nlm.nih.gov/9558116/
- https://pubmed.ncbi.nlm.nih.gov/12208882/
- https://pubmed.ncbi.nlm.nih.gov/15194813/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/20008295/
- https://pubmed.ncbi.nlm.nih.gov/21193407/
- https://pubmed.ncbi.nlm.nih.gov/27055590/
- https://pubmed.ncbi.nlm.nih.gov/28807980/
- https://pubmed.ncbi.nlm.nih.gov/7867070/
- https://pubmed.ncbi.nlm.nih.gov/8747460/
- https://pubmed.ncbi.nlm.nih.gov/9687375/
- https://pubmed.ncbi.nlm.nih.gov/9560195/
- https://pubmed.ncbi.nlm.nih.gov/18204448/
- https://pubmed.ncbi.nlm.nih.gov/18204446/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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