Nourseothricin (sulfate) [96736-11-7]
Cat# HY-129065-1ml
Size : 10mM/1mL
Brand : MedChemExpress
Nourseothricin sulfate (Streptothricin sulfate) is a broad-spectrum antibiotic that destroys the outer membrane of Gram-negative bacteria and is a dominant selective marker for Fonsecaea pedrosoi. Nourseothricin sulfate inhibits protein biosynthesis in prokaryotic cells and strongly inhibits the growth of eukaryotes like fungi and can also be used as a elective marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells.
For research use only. We do not sell to patients.
Nourseothricin sulfate Chemical Structure
CAS No. : 96736-11-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
Nourseothricin sulfate (Streptothricin sulfate) is a broad-spectrum antibiotic that destroys the outer membrane of Gram-negative bacteria and is a dominant selective marker for Fonsecaea pedrosoi[1][2]. Nourseothricin sulfate inhibits protein biosynthesis in prokaryotic cells and strongly inhibits the growth of eukaryotes like fungi and can also be used as a elective marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells[3]. |
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In Vitro |
Escherichia coli resistance to neurosporin can be sensitive to resistant strains by eliminating their outer membrane resistance. The polycationic antibiotic, Nourseothricin, represents a mixture of several Streptothricins, mainly D and F. Obviously, although very slowly, it can pass the outer membrane via the porin pores. It has been shown earlier that Nourseothricin is able to generate some kind of channels into the outer membrane through which it can pass the cell wall. On the other hand, there are indications that resistant strains containing a Nourseothricin-inactivating acetyl transferase possess an additional protecting system, namely a reduced penetrability of the outer membrane[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Nourseothricin is preferentially excreted via kidney and signs of nephrotoxicity can be observed after its administration. Renal handling of Nourseothricin is characterized in experiments on renal cortical slices under various experimental conditions. Following administration in vivo the renal tubular transport system for organic anions (p-aminohippurate, PAH) is not influenced by Nourseothricin. There is a high degree of accumulation of Nourseothricin in renal cortical slices. In contrast to PAH accumulation there is no influence of nitrogen atmosphere, simultaneous administration of PAH, probenecid or trishydroxyaminomethane on Nourseothricin accumulation. Age dependent differences in Nourseothricin accumulation does not exist[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
1359.47 |
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Formula |
C50H94N20O22S |
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CAS No. |
96736-11-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
NC(O[C@H]1[C@@H](CO)O[C@@H](NC(N2)=N[C@]([C@@]2(03)[C@H](O)CN3)(03)C3=O)[C@H](NC(C[C@@H](N)CCCN(C(C[C@@H](N)CCCNC(C[C@@H](N)CCCN)=O)=O)03)=O)[C@@H]1O)=O.O=S(O)(O)=O.NC(O[C@H]4[C@@H](CO)O[C@@H](NC(N5)=N[C@]([C@@]5(03)[C@H](O)CN6)(03)C6=O)[C@H](NC(C[C@@H](N)CCCN)=O)[C@@H]4O)=O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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Solvent & Solubility |
In Vitro:
H2O : ≥ 100 mg/mL (73.56 mM) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: ≥99.0% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O | 1 mM | 0.7356 mL | 3.6779 mL | 7.3558 mL | 18.3895 mL |
5 mM | 0.1471 mL | 0.7356 mL | 1.4712 mL | 3.6779 mL | |
10 mM | 0.0736 mL | 0.3678 mL | 0.7356 mL | 1.8390 mL | |
15 mM | 0.0490 mL | 0.2452 mL | 0.4904 mL | 1.2260 mL | |
20 mM | 0.0368 mL | 0.1839 mL | 0.3678 mL | 0.9195 mL | |
25 mM | 0.0294 mL | 0.1471 mL | 0.2942 mL | 0.7356 mL | |
30 mM | 0.0245 mL | 0.1226 mL | 0.2452 mL | 0.6130 mL | |
40 mM | 0.0184 mL | 0.0919 mL | 0.1839 mL | 0.4597 mL | |
50 mM | 0.0147 mL | 0.0736 mL | 0.1471 mL | 0.3678 mL | |
60 mM | 0.0123 mL | 0.0613 mL | 0.1226 mL | 0.3065 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.