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Mouse Monoclonal Anti-Ferritin, Light Chain (FTL) (Microglia Marker) [Clone FTL/1389]
Cat# NB-36-00211-P1
Size : 100ug
Brand : Neo Biotech
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Printable Datasheet
Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) Antibody [FTL/1389]
Catalog Number Formulation Size Price
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Western Blot Analysis of human HeLa cell lysate using Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1389).
SDS-PAGE Analysis of Purified Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1389). Confirmation of Integrity and Purity of Antibody
Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1389) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of kidney tissue lysate using Ferritin, Light Chain Monoclonal Antibody (FTL/1389).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immuno Oncology, Immunology, Dendritic Cell Marker
Isotype / Light Chain
IgG1 / Kappa
Gene Name
FTL
Positive Control
Cerebellum or Testis., HeLa, HePG2, HL-60 or 293T cells. Pancreas, Kidney, Liver
Immunogen
Recombinant fragment (around aa 38-165) of human FTL protein (exact sequence is proprietary)
Alternate Names
Ferritin light chain, Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; FTL; LFTD; NBIA3
Functions
- Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
Key References
- Y.Kaneko,T.Kitamoto,J.Tateishi,K.Yamaguchi. Ferritin immunohistochemistry as a marker for microglia. Acta Neuropathologica. 1989,Volume 79; 129-136
- Jezequel P, et. al. Validation of tumor-associated macrophage ferritin light chain as a prognostic biomarker in node-negative breast cancer tumors: A multicentric 2004 national PHRC study Int. J Cancer. 2012;131(2):426-37
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/3840162/
- https://pubmed.ncbi.nlm.nih.gov/3858810/
- https://pubmed.ncbi.nlm.nih.gov/3754330/
- https://pubmed.ncbi.nlm.nih.gov/14702039/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/15057824/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/3023856/
- https://pubmed.ncbi.nlm.nih.gov/6653779/
- https://pubmed.ncbi.nlm.nih.gov/8706699/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/23940258/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/25944712/
- https://pubmed.ncbi.nlm.nih.gov/16790936/
- https://pubmed.ncbi.nlm.nih.gov/19923220/
- https://pubmed.ncbi.nlm.nih.gov/20159981/
- https://pubmed.ncbi.nlm.nih.gov/16116125/
- https://pubmed.ncbi.nlm.nih.gov/19176363/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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