Images
Western Blot
Application

Western Blot

Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Biotin) (Cat # MAB14932) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (lambda).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse immunoglobulin lambda light chain.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgM lambda.

    Sequence

    N/A

    Reactivity

    Mouse

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.005-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Biotin) (Cat # MAB14932) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (lambda).

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — Igl-C1

    Entrez GeneID

    110785

    Protein Accession#

    P01843

    Gene Name

    Igl-C1

    Gene Alias

    Clambda1

    Gene Description

    immunoglobulin lambda chain, constant region 1

    Gene Ontology

    Hyperlink

    Gene Summary

    O

    Other Designations

    -