MeWo
Cat# 300285
Size : 1cryovial
Brand : CLS Cell Lines Service
MeWo Cells
General information
Description | The cells support the growth of varicella-zoster virus (VZV) isolates at 36 degree Celsius although growth is optimal at 32 degree Celsius. |
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Organism | Human |
Tissue | Skin |
Disease | Cutaneous melanoma |
Metastatic site | Lymph node |
Applications | Virus studies |
Synonyms | MEWO, Mewo, Me Wo, Me-Wo, Mevo, SK-MEL-MeWo, Mel-MeWo, BI-Mel, EST50 |
Characteristics
Age | 78 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | MeWo (Cytion catalog number 300285) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Forms malignant melanoma |
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Products | Melanin |
Mutational profile | BRAF V600E wt |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 12 D13S317: 8,9 D16S539: 10,12 D5S818: 12,13 D7S820: 10,12 TH01: 7,9 TPOX: 8,1 vWA: 15 D3S1358: 17 D21S11: 30,32.2 D18S51: 14,17 Penta E: 5 Penta D: 10 D8S1179: 13,15 FGA: 22 D1S1656: 15,16 D6S1043: 12 D2S1338: 21,23 D12S391: 16,17 D19S433: 14,16 |
HLA alleles | A*: 02:01:01, 26:01:01 B*: 14:02:01, 38:01:01 C*: 08:02:01, 12:03:01 DRB1*: 01:02:01, 11:01:01G DQA1*: 01:01:02, 05:05:01 DQB1*: 03:01:01G, 05:01:01G DPB1*: 04:01:01G, 04:02:01G E: 01:xx, 01:03:01 |