Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (PE)

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.||Applications:|Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.|Immunohistochemistry (Frozen and Paraffin): Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.|Optimal working dilutions to be determined by researcher.||Recommended Positive Control:|Human spleen||Recommended Negative Control: |I1904-78R: IgG1: Murine Negative Control||Recommended Secondary Antibodies:|I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo|I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo|I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.