Ku, p70 (BSA & Azide Free) (FITC)

The Ku autoantigen is a heterodimer of 70kD (p70) and ~80kD (p80) proteins. The p70/p80 dimer is important for function of a 460kD DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. ||MW of Antigen: 70kD ||Epitope: aa506-541||Applications and Recommended Dilutions: |Immunofluorescence |Immunoprecipitation (Native and denatured) (Use Protein A) (Ab 2ug/mg protein lysate) |Western Blotting (0.25-0.5ug/ml for 2hrs at RT) |Immunohistochemistry (Formalinparaffin) (Ab 1-2ug/ml for 30 minute at RT) * [Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. ||Positive Control: BT474, T47D, HEP-G-2 or HeLa cells. Tonsil ||Cellular Localization: Nuclear||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note: Applications are based on unconjugated antibody.